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rptec growth kit components  (ATCC)


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    ATCC rptec growth kit components
    Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells <t>(RPTEC)</t> with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.
    Rptec Growth Kit Components, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htert+immortalized+rptec+growth+kit+components/hTERT+Immortalized+RPTEC+Growth+Kit/pmc13078729-89-32-36
    Average 95 stars, based on 74 article reviews
    rptec growth kit components - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "The cardiac circadian clock regulates rhythms in peripheral tissues via Fibulin 5"

    Article Title: The cardiac circadian clock regulates rhythms in peripheral tissues via Fibulin 5

    Journal: Comprehensive Physiology

    doi: 10.1002/cph4.70147

    Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.
    Figure Legend Snippet: Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.

    Techniques Used: Recombinant, Gene Expression, Expressing

    Related Articles

    Cell Culture:

    Article Title: Mitochondrial toxicity of emerging disinfection byproduct 2,6-dichloro-1,4- benzoquinone in renal proximal tubule cells: Oxidative phosphorylation impairment and metabolic reprogramming.
    Article Snippet: 2,6-Dichloro-1,4-benzoquinone (DCBQ), an emerging aromatic disinfection byproduct (DBP) frequently detected in drinking water, has raised increasing concern regarding its potential renal toxicity.. However, its toxic effects at environmentally relevant concentrations and the underlying mechanisms remain unclear.. In this study, human renal proximal tubule epithelial cells (RPTEC/TERT1) were exposed to intrarenal-equivalent levels of DCBQ (10, 100, and 1000 μg/L) to investigate mitochondrial toxicity under a worst-case exposure scenario.

    Modification:

    Article Title: Mitochondrial toxicity of emerging disinfection byproduct 2,6-dichloro-1,4- benzoquinone in renal proximal tubule cells: Oxidative phosphorylation impairment and metabolic reprogramming.
    Article Snippet: 2,6-Dichloro-1,4-benzoquinone (DCBQ), an emerging aromatic disinfection byproduct (DBP) frequently detected in drinking water, has raised increasing concern regarding its potential renal toxicity.. However, its toxic effects at environmentally relevant concentrations and the underlying mechanisms remain unclear.. In this study, human renal proximal tubule epithelial cells (RPTEC/TERT1) were exposed to intrarenal-equivalent levels of DCBQ (10, 100, and 1000 μg/L) to investigate mitochondrial toxicity under a worst-case exposure scenario.



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    Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells <t>(RPTEC)</t> with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.
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    https://www.bioz.com/product/htert+immortalized+rptec+growth+kit+components/hTERT+Immortalized+RPTEC+Growth+Kit/pmc13078729-89-32-36
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    htert immortalized rptec growth kit components  (ATCC)
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    Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells <t>(RPTEC)</t> with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.
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    Image Search Results


    Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.

    Journal: Comprehensive Physiology

    Article Title: The cardiac circadian clock regulates rhythms in peripheral tissues via Fibulin 5

    doi: 10.1002/cph4.70147

    Figure Lengend Snippet: Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.

    Article Snippet: The human Renal Proximal Tubule Epithelial Cell (RPTEC) immortalized cell line (ATCC, PCS-400–010) was cultured in complete growth medium consisting of the base medium containing DMEM: F12 Medium (ATCC 30–2006) and the RPTEC Growth Kit components (ATCC ACS-4007) consisting of 5 pM triiodo-L-thyronine, 10 ng/mL recombinant human EGF, 3.5 μg/mL ascorbic acid, 5.0 μg/mL human transferrin, 5.0 μg/mL insulin, 25 ng/mL prostaglandin E 1 , 25 ng/mL hydrocortisone, 8.65 ng/mL sodium selenite and 1.2 mg/mL sodium bicarbonate.

    Techniques: Recombinant, Gene Expression, Expressing